A fidget spinner for the point-of-care diagnosis of urinary tract infection.
Issac MichaelDongyoung KimOleksandra GulenkoSumit KumarSaravana KumarJothi ClaraDong Yeob KiJuhee ParkHyun Yong JeongTaek Soo KimSunghoon KwonYoon-Kyoung ChoPublished in: Nature biomedical engineering (2020)
The point-of-care detection of pathogens in biological samples in resource-limited settings should be inexpensive, rapid, portable, simple and accurate. Here, we describe a custom-made fidget spinner that rapidly concentrates pathogens in 1-ml samples of undiluted urine by more than 100-fold for the on-device colorimetric detection of bacterial load and pathogen identification. In Tiruchirappalli, India, the device enabled the on-site detection of infection with the naked eye within 50 min in urine samples from 39 patients suspected of having a urinary tract infection. We also show that, in 30 clinical samples of urinary tract infection, the device can be used to perform an antimicrobial susceptibility test for the antimicrobial drugs ciprofloxacin and cefazolin within 120 min. The fidget spinner could be used in low-resource settings as an inexpensive handheld point-of-care device for the rapid concentration and detection of pathogens in urine samples.
Keyphrases
- urinary tract infection
- loop mediated isothermal amplification
- label free
- sensitive detection
- real time pcr
- gram negative
- end stage renal disease
- newly diagnosed
- antimicrobial resistance
- gold nanoparticles
- chronic kidney disease
- ejection fraction
- prognostic factors
- high resolution
- cystic fibrosis
- fluorescent probe
- quantum dots
- aqueous solution
- bioinformatics analysis