The Efficacy of Transplanting Human Umbilical Cord Mesenchymal Stem Cell Sheets in the Treatment of Myocardial Infarction in Mice.
Thang Quoc BuiNguyen Trong BinhTruc Le-Buu PhamTrinh Van LeNhung Hai TruongDang Phu-Hai NguyenThao Thi-Thu LuuTrang Nguyen-Xuan PhamTu Cam TranHuyen Thi-Thuong NguyenNhu Thuy-TrinhPhong Anh TranPublished in: Biomedicines (2023)
The transplantation of mesenchymal stem cell (MSC) sheets derived from human umbilical cords (hUCs) was investigated in this study as a potential application in treating myocardial infarction (MI). Two groups of hUC-MSC sheets were formed by populating LunaGel TM , which are 3D scaffolds of photo-crosslinkable gelatin-based hydrogel with two different cell densities. An MI model was created by ligating the left anterior descending coronary artery of healthy BALB/c mice. After two weeks, the cell sheets were applied directly to the MI area and the efficacy of the treatment was evaluated over the next two weeks by monitoring the mice's weight, evaluating the left ventricle ejection fraction, and assessing the histology of the heart tissue at the end of the experiment. Higher cell density showed significantly greater efficiency in MI mice treatment in terms of weight gain and the recovery of ejection fraction. The heart tissue of the groups receiving cell sheets showed human-CD44-positive staining and reduced fibrosis and apoptosis. In conclusion, the hUC-MSC sheets ameliorated heart MI injury in mice and the efficacy of the cell sheets improved as the number of cells increased.
Keyphrases
- ejection fraction
- cell therapy
- single cell
- mesenchymal stem cells
- heart failure
- umbilical cord
- weight gain
- coronary artery
- high fat diet induced
- endothelial cells
- stem cells
- left ventricular
- cell cycle arrest
- physical activity
- atrial fibrillation
- type diabetes
- oxidative stress
- coronary artery disease
- pulmonary hypertension
- mass spectrometry
- wild type
- insulin resistance
- hyaluronic acid
- endoplasmic reticulum stress
- cell proliferation
- drug delivery
- climate change
- skeletal muscle
- adipose tissue
- signaling pathway
- preterm birth
- birth weight
- smoking cessation
- bone regeneration