Quality Control, Anti-Hyperglycemic, and Anti-Inflammatory Assessment of Colvillea racemosa Leaves Using In Vitro, In Vivo Investigations and Its Correlation with the Phytoconstituents Identified via LC-QTOF-MS and MS/MS.
Mohamed S Abd El HafeezOmayma El GindiMona H HettaHanan F AlySafwat A AhmedPublished in: Plants (Basel, Switzerland) (2022)
Colvillea racemosa is a cultivated ornamental plant that is a monotypic genus of Fabaceae. It is native to Madagascar, with limited studies. For the first time, the leaf quality control parameters, the anti-hyperglycemic and anti-inflammatory in vitro activity of Colvillea racemosa ethanol extract (CRE) and its fractions of petroleum ether (CRP), methylene chloride (CRMC), ethyl acetate (CREA), n -butanol (CRB), and methanol (CRME) were evaluated. It exhibited significant inhibition against α-amylase, α-glucosidase and membrane stabilization. CRB was the most active fraction, and in vivo studies revealed that oral treatment with CRB of STZ-induced diabetic rats efficiently lowered blood glucose by 67.78%, reduced serum nitric oxide and lipid peroxide levels by 41.23% and 38.45%, respectively, and increased the GSH level by 90.48%. In addition, compared with the diabetic group, there was a 52.2% decrease in serum VCAM, a 55.5% increase in paraoxonase, an improved lipid profile, and improved liver and kidney functions for a treated diabetic group with CRB. Metabolite profiling of CRB was determined by UPLC-ESI-QTOF-MS and tandem MS/MS. Twenty-three chromatographic peaks were identified, which were classified into phenolic compounds and amino acids. The characterized flavonoids were apigenin and luteolin derivatives.
Keyphrases
- ms ms
- quality control
- diabetic rats
- anti inflammatory
- oxidative stress
- blood glucose
- nitric oxide
- simultaneous determination
- liquid chromatography tandem mass spectrometry
- type diabetes
- amino acid
- single cell
- mass spectrometry
- ultra high performance liquid chromatography
- ionic liquid
- wound healing
- case control
- blood pressure
- hydrogen peroxide
- high performance liquid chromatography
- high glucose
- skeletal muscle
- liquid chromatography
- endothelial cells
- multiple sclerosis
- high resolution
- weight loss
- high resolution mass spectrometry