Revisit PEG-Induced Precipitation Assay for Protein Solubility Assessment of Monoclonal Antibody Formulations.

Martha J ScannellMatthew W HyattIvan L BudyakMahlet A WoldeyesYing Wang

Published in: Pharmaceutical research (2021)

This study demonstrated good correlations between the protein solubility measures obtained from PEG-induced precipitation experiments and DLS kD measurement. It also provides one example in which protein solubility ranking by binding free energy is more accurate than the other measures. The results support the theoretical proposition that μB has a potential to serve as standard protein solubility measure.

Keyphrases

monoclonal antibody
protein protein
amino acid
high glucose
drug delivery
diabetic rats
high throughput
oxidative stress
endothelial cells
risk assessment
drug induced
climate change
single cell