Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil.
Vanessa Silva-MoraesLisa Marie ShollenbergerWilliam Castro-BorgesAna Lucia Teles RabelloDonald A HarnLia Carolina Soares MedeirosWander de Jesus JeremiasLiliane Maria Vidal SiqueiraCaroline Stephane Salviano PereiraMaria Luysa Camargos PedrosaNathalie Bonatti Franco AlmeidaAureo AlmeidaJose Roberto LambertucciNídia Francisca de Figueiredo CarneiroPaulo Marcos Zech CoelhoRafaella Fortini Queiroz GrenfellPublished in: PLoS neglected tropical diseases (2019)
The serological proteome approach was able to identify a new diagnostic candidate. The recombinant egg antigen provided good performance in IgG-ELISA to detect individuals with extreme low-intensity infections (1 egg per gram of feces). Therefore, the IgG-ELISA using this newly identified recombinant MEA can be a useful tool combined with other techniques in low-endemic areas to determine the true prevalence of schistosome infection that is underestimated by the KK method. Further, to overcome the complexity of ELISA in the field, a second generation of antibody-based rapid diagnostic tests (RDT) can be developed.