Preparation of a highly specific single chain variable fragment antibody targeting miroestrol and its application in quality control of Pueraria candollei by enzyme-linked immunosorbent assay.
Benyakan PongkitwitoonPanitch BoonsnongcheepTharita KitisripanyaGorrawit YusakulSeiichi SakamotoHiroyuki TanakaSatoshi MorimotoWaraporn PutalunPublished in: Phytochemical analysis : PCA (2019)
The developed scFv-based ELISA was sensitive, specific, accurate, and precise for determination of miroestrol and useful for quality control of P. candollei plant raw materials and supplementary products.