Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR.

Claire Y T WangJames S McCarthyWill J StoneTeun BousemaKatharine A CollinsSeweryn Bialasiewicz

Published in: Malaria journal (2018)

This semi-automated qPCR method enables accurate detection of low-level P. falciparum oocyst infections in mosquito midguts, and may improve the sensitivity, specificity and throughput of assays used to evaluate candidate transmission-blocking interventions.

Keyphrases

plasmodium falciparum
high throughput
aedes aegypti
dengue virus
real time pcr
high resolution
machine learning
zika virus
physical activity
deep learning
loop mediated isothermal amplification
quantum dots