Trans-segmental imaging in the spinal cord of behaving mice.
Pavel ShekhtmeysterDaniela DuarteErin M CareyAlexander NgoGrace GaoJack A OlmsteadNicholas A NelsonAxel NimmerjahnPublished in: Nature biotechnology (2023)
Spinal cord circuits play crucial roles in transmitting pain, but the underlying activity patterns within and across spinal segments in behaving mice have remained elusive. We developed a wearable widefield macroscope with a 7.9-mm 2 field of view, ~3- to 4-μm lateral resolution, 2.7-mm working distance and <10-g overall weight and show that highly localized painful mechanical stimuli evoke widespread, coordinated astrocyte excitation across multiple spinal segments.
Keyphrases
- spinal cord
- neuropathic pain
- spinal cord injury
- high fat diet induced
- chronic pain
- high resolution
- body mass index
- physical activity
- pain management
- weight loss
- insulin resistance
- single molecule
- type diabetes
- adipose tissue
- weight gain
- metabolic syndrome
- skeletal muscle
- mass spectrometry
- quantum dots
- postoperative pain