A novel method to rescue and culture duck Astrovirus type 1 in vitro.

Ruihua ZhangJingjing LanHaie LiJunhao ChenYupeng YangShaoli LinZhijing XieShi-Jin Jiang

Published in: Virology journal (2019)

An improved DNA-launched reverse genetics system for DAstV-1 was firstly constructed. Infectious virus recovered from BHK-21 cells could propagate in DEF cells pre-treated with trypsin. This is the first report of the successful in vitro cultivation of DAstV-1. We believe this valuable experimental system will contribute to the further study of DAstV-1 genome function and pathogenesis.

Keyphrases

induced apoptosis
cell cycle arrest
endoplasmic reticulum stress
oxidative stress
signaling pathway
wastewater treatment
dna methylation
cell proliferation
genome wide
cell free