Influence of Carboxyl Group Ratios on the Design of Breast Cancer Targeting Bimodal MR/NIR-II Imaging Probe from PLGA@Gd-DOTA@PEG Micelles Conjugating Herceptin.
Thi Kim Dung DoanMasakazu UmezawaIkeda KazunoKazunobu OhnukiMizuki AkatsukaTakanori IchikiMasao KamimuraMasayuki YamaguchiHirofumi FujiiKohei SogaPublished in: ACS applied bio materials (2023)
We developed a small MRI/NIR-II probe to target HER2 (tetanucleotide) breast cancer cells. The probe is composed of PLGA- b -PEG micelles encapsulated NIR-II, and Gd-DOTA is conjugated at the border of PLGA/PEG. Herceptin was then conjugated to carboxyl residues of PLGA- b -PEG chains. We examined the influence of carboxyl group ratios on the probe property stability and Herceptin concentration and the binding affinity to HER2(+) cells corresponding to the -COOH ratios. The binding assays demonstrated that the optimal surface ratio of -COOH is 5%, which is less affected by fluorescence reduction and which exhibited the highest antigen-capturing activity.
Keyphrases
- drug delivery
- drug release
- cancer therapy
- living cells
- photodynamic therapy
- fluorescent probe
- quantum dots
- breast cancer cells
- pet imaging
- pet ct
- induced apoptosis
- single molecule
- high resolution
- magnetic resonance imaging
- cell cycle arrest
- oxidative stress
- high throughput
- young adults
- computed tomography
- dna binding
- mass spectrometry
- capillary electrophoresis
- signaling pathway
- transcription factor