Azacoumarin-based "turn-on" fluorescent probe for the detection and imaging of hydrogen peroxide in living cells.
Samarpita DasAiswarya ChaudhuriHarish K IndurthiAshish K AgrawalDeepak K SharmaPublished in: Organic & biomolecular chemistry (2024)
Azacoumarins are a relatively unexplored group of coumarin fluorophores, despite their excellent light-emitting properties. In this report, we detail the creation and production of a fluorescent probe (PYCB) based on azacoumarin for detecting H 2 O 2 . The probe utilizes a carboxy benzyl boronic pinacol ester as the recognition unit and displays a turn-on fluorescence response at 460 nm upon exposure to H 2 O 2 . The probe shows excellent sensitivity and selectivity to H 2 O 2 , with a detection limit of 0.385 μM. PYCB also exhibited strong pH stability and selectivity for H 2 O 2 over other reactive oxygen species (ROS). Additionally, MTT assay results demonstrated the excellent biocompatibility of PYCB in MCF-7 cell lines. Fluorescence imaging of PYCB-treated MCF-7 cells revealed enhanced blue fluorescence corresponding to varying concentrations of exogenous H 2 O 2 .
Keyphrases
- living cells
- fluorescent probe
- light emitting
- hydrogen peroxide
- fluorescence imaging
- reactive oxygen species
- photodynamic therapy
- single molecule
- breast cancer cells
- induced apoptosis
- nitric oxide
- loop mediated isothermal amplification
- real time pcr
- label free
- high resolution
- cell cycle arrest
- cell death
- dna damage
- energy transfer
- high throughput
- cell proliferation
- pi k akt