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Engineering xylose metabolism in thraustochytrid T18.

Alexandra Merkx-JacquesHolly RasmussenDenise M MuiseJeremy J R BenjaminHaila KottwitzKaitlyn TannerMichael T MilwayLaura M PurdueMark A ScaifeRoberto E ArmentaDavid L Woodhall
Published in: Biotechnology for biofuels (2018)
The presence of a typically prokaryotic xylose isomerase and xylitol production through a typically eukaryotic xylose reductase pathway in T18 is the first report of an organism naturally encoding enzymes from two native xylose metabolic pathways. Our newly engineered strains pave the way for the growth of T18 on waste hemicellulosic feedstocks for biofuel production.
Keyphrases
  • saccharomyces cerevisiae
  • escherichia coli
  • heavy metals
  • risk assessment