Efficient dispersive solid-phase extraction of methylprednisolone from exhaled breath of COVID-19 patients.
Yasaman Sefid-SefidehkhanMehdi MokhtariAta MahmoodpoorYosra Vaez-GharamalekiMaryam KhoubnasabjafariMohammad Reza Afshar MoghaddamVahid Jouyban-GharamalekiSiavoush DastmalchiAlireza ForoumadiAbolghasem JouybanPublished in: RSC advances (2023)
In the current study, bismuth ferrite nano-sorbent was synthesized and utilized as a sorbent for the dispersive solid-phase extraction of methylprednisolone from exhaled breath samples. The size and morphology of the nano-sorbent were characterized by X-ray diffraction analysis and scanning electron microscopy. Following its desorption with acetonitrile, methylprednisolone was quantified by a high-performance liquid chromatography-ultraviolet detector. Factors affecting the extraction of methylprednisolone were optimized. Under optimized experimental conditions, a linear relationship between the analytical signals and methylprednisolone concentration was obtained in the range of 0.001-0.2 μg mL -1 for exhaled breath condensate samples and 0.002-0.4 μg per filter for filter samples. A pre-concentration factor of 6.4-fold, corresponding to an extraction recovery of 96.0%, was achieved. The validated method was applied for the determination of methylprednisolone in real samples taken from the exhaled breath of COVID-19 patients under mechanical ventilation.
Keyphrases
- solid phase extraction
- high performance liquid chromatography
- electron microscopy
- high dose
- molecularly imprinted
- liquid chromatography tandem mass spectrometry
- liquid chromatography
- tandem mass spectrometry
- gas chromatography mass spectrometry
- simultaneous determination
- mechanical ventilation
- ultra high performance liquid chromatography
- gas chromatography
- sars cov
- low dose
- mass spectrometry
- intensive care unit
- acute respiratory distress syndrome
- high resolution
- respiratory failure
- magnetic resonance imaging
- high resolution mass spectrometry