Compositionally unique mitochondria in filopodia support cellular migration.
Madeleine Marlar-PaveyDaniel Tapias-GomezMarcel MettlenJonathan R FriedmanPublished in: bioRxiv : the preprint server for biology (2024)
Local metabolic demand within cells varies widely and the extent to which individual mitochondria can be specialized to meet these functional needs is unclear. We examined the subcellular distribution of MICOS, a spatial and functional organizer of mitochondria, and discovered that it dynamically enriches at the tip of a minor population of mitochondria in the cell periphery that we term METEORs. METEORs have a unique composition; MICOS enrichment sites are depleted of mtDNA and matrix proteins and contain high levels of the Ca2+ uniporter MCU, suggesting a functional specialization. METEORs are also enriched for the myosin MYO19, which promotes their trafficking to a small subset of filopodia. We identify a positive correlation between the length of filopodia and the presence of METEORs and show that elimination of mitochondria from filopodia impairs cellular motility. Our data reveal a novel type of mitochondrial heterogeneity and suggest compositionally specialized mitochondria support cell migration.
Keyphrases
- cell death
- endoplasmic reticulum
- reactive oxygen species
- cell migration
- single cell
- palliative care
- cell cycle arrest
- induced apoptosis
- oxidative stress
- preterm infants
- gene expression
- cystic fibrosis
- mitochondrial dna
- signaling pathway
- electronic health record
- copy number
- mesenchymal stem cells
- cell proliferation
- binding protein
- bone marrow
- endoplasmic reticulum stress