Ochrasperfloroid, an ochratoxin-ergosteroid heterodimer with inhibition of IL-6 and NO production from Aspergillus flocculosus 16D-1.
Bin-Bin GuFu-Rong JiaoWei WuLei LiuHou-Wen LinFan SunShu-Ping WangFan YangHou-Wen LinPublished in: RSC advances (2019)
A novel ochratoxin-ergosteroid heterodimer, ochrasperfloroid (1), together with a known mycotoxin, ochratoxin A (2), were isolated from the sponge-derived fungus Aspergillus flocculosus 16D-1. The structure of 1 was determined on the basis of 1D/2D NMR, HRESIMS/MS, and LC-UV/MS analysis of its alkaline hydrolyzates, quantum-chemical 13 C NMR calculation, and comparison with literature data. Of note, the ergosteroid embedded in 1 is also a new structure. Ochrasperfloroid (1) showed potent inhibitory activity towards IL-6 production in lipopolysaccharide (LPS)-induced THP-1 cell line, with an IC 50 value of 2.02 μM, and NO production in LPS-activated RAW264.7 macrophages, with an IC 50 value of 1.11 μM.
Keyphrases
- lps induced
- inflammatory response
- mass spectrometry
- magnetic resonance
- high resolution
- multiple sclerosis
- ms ms
- solid state
- toll like receptor
- systematic review
- anti inflammatory
- electronic health record
- liquid chromatography
- cell wall
- big data
- simultaneous determination
- immune response
- machine learning
- gas chromatography
- data analysis
- energy transfer
- deep learning
- aqueous solution
- artificial intelligence