Transcriptional dynamics of granulocytes in direct response to incubation with SARS-CoV-2.
Daigo NakazawaYohei TakedaMasatoshi KandaUtano TomaruHaruko OgawaTakashi KudoSatoka Shiratori-AsoKanako Watanabe-KusunokiYusho UedaAtsuko MiyoshiFumihiko HattandaSaori NishioRyo UozumiAkihiro IshizuTatsuya AtsumiPublished in: FEBS open bio (2022)
Severe coronavirus disease 2019 (COVID-19) is characterized by acute respiratory distress syndrome and multiple organ dysfunction, in which the host immune response plays a pivotal role. Excessive neutrophil activation and subsequent superfluity of neutrophil extracellular traps (NETs) can lead to tissue damage, and several studies have shown the involvement of neutrophils in severe COVID-19. However, the detailed responses of each neutrophil subset to SARS-CoV-2 infection has not been fully described. To explore this issue, we incubated normal-density granulocytes (NDGs) and low-density granulocytes (LDGs) with different viral titers of SARS-CoV-2. NDGs form NETs with chromatin fibers in response to SARS-CoV-2, whereas LDGs incubated with SARS-CoV-2 display a distinct morphology with condensed nuclei and moderate transcriptional changes. Based on these transcriptional changes, we suggest that AGO2 possibly plays a role in LDG regulation in response to SARS-CoV-2.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- coronavirus disease
- acute respiratory distress syndrome
- gene expression
- transcription factor
- immune response
- extracorporeal membrane oxygenation
- oxidative stress
- mechanical ventilation
- early onset
- heat shock
- weight gain
- dna methylation
- physical activity
- dendritic cells
- drug induced
- heat shock protein