A rapid real-time polymerase chain reaction-based live virus microneutralization assay for detection of neutralizing antibodies against SARS-CoV-2 in blood/serum.

Syed Hani AbidiKehkashan ImtiazAkbar KanjiShama QaiserErum KhanKiran IqbalMarc VeldhoenKulsoom GhiasJ Pedro SimasZahra Hasan

Published in: PloS one (2021)

We describe a rapid RT-PCR-based SARS-CoV-2 microneutralization assay for the detection of neutralizing antibodies. This can effectively be used to test the antiviral activity of serum antibodies for the investigation of both disease-driven and vaccine-induced responses.

Keyphrases

sars cov
loop mediated isothermal amplification
real time pcr
respiratory syndrome coronavirus
high throughput
sensitive detection
dengue virus
label free
high glucose
oxidative stress