The impact of long-term storage conditions on the development of experimental dentinal microcracks.
Gustavo De-DeusFabio Nakao ArashiroErick Miranda SouzaEmmanuel João Nogueira Leal da SilvaTiago de Mello GuimarãesAna Carolina de Carvalho MacielDiogo da Silva OliveiraFelipe Gonçalves BelladonnaRicardo Tadeu LopesMarco Aurélio VersianiPublished in: Australian endodontic journal : the journal of the Australian Society of Endodontology Inc (2023)
This study evaluated the impact of long-term storage conditions (medium and time) on the development of experimental dentinal microcracks through micro-computed tomography. Sixty freshly extracted premolars were stored in formalin, water, or dry conditions (n = 20) and scanned after 72 h, 30 days, 6 months and 3 years of extraction. The effect of the storage medium and time on the occurrence of dentinal defects was statistically evaluated. A total of 211 000 images were screened revealing the existence of 11 519 slices with dentinal defects. Dry conditions significantly contributed to the development of new defects in all time points. During the 3-year follow-up period, no new defects were detected in the teeth that were stored in water and, in a single tooth, in the formalin group, after 6 months of storage.