Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water".

Robert B BestWenwei ZhengAlessandro Borgia Karin J Buholzer Madeleine B BorgiaHagen HofmannAndrea SorannoDaniel NettelsKlaus GastAlexander V Grishaev Benjamin Schuler

Published in: Science (New York, N.Y.) (2018)

Riback et al (Reports, 13 October 2017, p. 238) used small-angle x-ray scattering (SAXS) experiments to infer a degree of compaction for unfolded proteins in water versus chemical denaturant that is highly consistent with the results from Förster resonance energy transfer (FRET) experiments. There is thus no "contradiction" between the two methods, nor evidence to support their claim that commonly used FRET fluorophores cause protein compaction.

Keyphrases

energy transfer
quantum dots
high resolution
ionic liquid
protein protein
mass spectrometry
small molecule
data analysis