Toxicological assessment of lambda-cyhalothrin and acetamiprid insecticides formulated mixture on hatchability rate, histological aspects, and protein electrophoretic pattern of Biomphalaria alexandrina (Ehrenberg, 1831) snails.
Amina Mohamed IbrahimSara S M SayedIbrahim R A ShalashPublished in: Environmental science and pollution research international (2018)
Several formulated mixtures of pesticides are widely used in modern agriculture. Nevertheless, the agriculture runoff causes a serious damage to the aquatic ecosystem. Therefore, the present study aims to use B. alexandrina snails as bioindicators for 30 g/l lambda-cyhalothrin and 17 g/l acetamiprid as a formulated mixture insecticide. Results showed that it has a molluscicidal activity against snails at LC50 7.9 mg/l. The hatchability percent of both treated 1-day-aged and/or 3-day-aged groups were less than that of the control group. The sublethal concentrations of the tested insecticide caused a remarkable abnormal necrosis in male and female gametogenic cells, besides a severe damage in both secretory and digestive cells. The results of SDS-PAGE protein profiles of treated snails showed that the least number of protein bands was noticed in snail groups that subjected to LC10 (6.6 mg/l) and LC25 (7.2 mg/l) concentrations when compared to control protein fractions.
Keyphrases
- induced apoptosis
- climate change
- oxidative stress
- risk assessment
- protein protein
- cell cycle arrest
- amino acid
- binding protein
- aedes aegypti
- simultaneous determination
- epithelial mesenchymal transition
- mass spectrometry
- signaling pathway
- cell proliferation
- zika virus
- cell death
- newly diagnosed
- tandem mass spectrometry
- high resolution mass spectrometry