The Evaluation of Eazyplex ® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures.
Alicja SękowskaTomasz BogielPublished in: Antibiotics (Basel, Switzerland) (2022)
Increasing antimicrobial resistance of Gram-negative rods is an important diagnostic, clinical and epidemiological problem of modern medicine. Therefore, it is important to detect multi-drug resistant strains as early on as possible. This study aimed to evaluate Eazyplex ® SuperBug CRE assay usefulness for beta-lactamase gene detection among Gram-negative rods, directly from urine samples and positive blood cultures. The Eazyplex ® SuperBug CRE assay is based on a loop-mediated isothermal amplification of genetic material and allows for the detection of a selection of genes encoding carbapenemases, KPC, NDM, VIM, OXA-48, OXA-181 and extended-spectrum beta-lactamases from the CTX-M-1 and CTX-M-9 groups. A total of 120 clinical specimens were included in the study. The test gave valid results for 58 (96.7%) urine samples and 57 (95.0%) positive blood cultures. ESBL and/or carbapenemase enzymes genes were detected in 56 (93.3%) urine and 55 (91.7%) blood samples, respectively. The Eazyplex ® SuperBug CRE assay can be used for a rapid detection of the genes encoding the most important resistance mechanisms to beta-lactams in Gram-negative rods also without the necessity of bacterial culture.
Keyphrases
- klebsiella pneumoniae
- multidrug resistant
- gram negative
- drug resistant
- loop mediated isothermal amplification
- acinetobacter baumannii
- genome wide
- genome wide identification
- antimicrobial resistance
- high throughput
- escherichia coli
- dna methylation
- bioinformatics analysis
- label free
- sensitive detection
- genome wide analysis
- real time pcr
- transcription factor
- copy number
- gene expression