In silico design and validation of a highly degenerate primer pair: a systematic approach.
Prosper Obed ChukwuemekaHaruna Isiyaku UmarOluwatoyin Folake OlukunleOluwaseyi Matthew OretadeChristopher Busayo OlowosokeEmmanuel Oluwasegun AkinsolaMichael Omoniyi ElabiyiUsman Garba KurmiJoy Oseme EigbeBukola Rukayat OyelereLucky Efe IsunuOyeyemi Janet OretadePublished in: Journal, genetic engineering & biotechnology (2020)
In this study, we provided a turnkey protocol to simplify the design of degenerate primers using the heuristics of the HYDEN software program.
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