Optimization of an ultra-bright real-time high-throughput renilla luciferase assay for antibacterial assessment of Streptococcus mutans biofilms.
Rochelle Denise HiersSharukh Soli KhajotiaJustin MerrittFernando Luis Esteban FlorezPublished in: Dental materials : official publication of the Academy of Dental Materials (2024)
The present study is anticipated to positively impact subsequent research on dental materials and oral microbiology because it serves as a valuable screening tool in metabolic-based assays with increased sensitivity and robustness. The assay reported is anticipated to be further optimized to be used as a co-reporter for other Luc based assays.