Electron tomography visualization of HIV-1 virions trapped by fusion inhibitors to host cells in infected tissues.
Mark S LadinskyLi ZhuIrfan UllahPradeep D UchilPriti KumarMichael S KayPamela J BjorkmanPublished in: bioRxiv : the preprint server for biology (2024)
Trapped and untrapped HIV-1 virions, both mature and immature, were distinguished by localizing spokes via 3D tomographic reconstructions of HIV-1 infected and fusion-inhibitor treated tissues of humanized mice. The finding of trapped HIV-1 virions in all tissues examined demonstrates a wide distribution of the CPT31 inhibitor, a desirable property for a potential therapeutic. In addition, the presence of virions trapped by spokes, particularly in vascular endothelial cells, demonstrates that fusion inhibitors can be used as markers for potential HIV-1-target cells within tissues, facilitating the mapping of HIV-1 target cells within the complex cellular milieu of infected tissues.
Keyphrases
- hiv infected
- antiretroviral therapy
- hiv positive
- human immunodeficiency virus
- hiv testing
- induced apoptosis
- hiv aids
- hepatitis c virus
- gene expression
- cell cycle arrest
- men who have sex with men
- endothelial cells
- endoplasmic reticulum stress
- type diabetes
- magnetic resonance imaging
- skeletal muscle
- cell death
- oxidative stress
- adipose tissue
- computed tomography
- cell proliferation
- risk assessment
- pi k akt