Cross-triggered and cascaded recycling amplification system for electrochemical detection of circulating microRNA in human serum.
Baoting DouHui ZhouYajun HongLiming ZhaoPo WangPublished in: Chemical communications (Cambridge, England) (2021)
A cross-triggered and cascaded recycling amplification system was developed for electrochemical sensing of microRNA 122 based on the DNAzyme/multicomponent nucleic acid enzyme cleavage technique and a dumbbell-shaped probe. The linear range and detection limit were obtained to be 1 fM-100 pM and 0.34 fM, respectively. Compared with some reported studies, the proposed system can achieve the selective detection of endogenous miRNA in liver injury patients and healthy human serums with the advantages of high sensitivity, low cost, and easy manipulation, which are significant for disease diagnosis as well as the fundamental research of molecular biology.
Keyphrases
- label free
- nucleic acid
- liver injury
- low cost
- drug induced
- loop mediated isothermal amplification
- ejection fraction
- end stage renal disease
- gold nanoparticles
- endothelial cells
- newly diagnosed
- living cells
- peritoneal dialysis
- single molecule
- quantum dots
- heavy metals
- molecularly imprinted
- transcription factor
- electron transfer
- simultaneous determination