Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.

Mariano CarossinoHon S IpJuergen A RichtKendra ShultzKimberly HarperAlan T LoynachanFabio Del PieroUdeni B R Balasuriya

Published in: Archives of virology (2020)

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

Keyphrases

respiratory syndrome coronavirus
sars cov
high throughput
coronavirus disease
cancer therapy
genome wide
gene expression
loop mediated isothermal amplification
label free