Engineering ketoreductases for the enantioselective synthesis of chiral alcohols.

Li QiaoZhiyuan LuoHaomin ChenPeng-Fei Zhang Anming Wang Roger A Sheldon

Published in: Chemical communications (Cambridge, England) (2023)

The use of engineered ketoreductases (KREDS), both as whole microbial cells and isolated enzymes, in the highly enantiospecific reduction of prochiral ketones is reviewed. The homochiral alcohol products are key intermediates in, for example, pharmaceuticals synthesis. The application of sophisticated protein engineering and enzyme immobilisation techniques to increase industrial viability are discussed.

Keyphrases

induced apoptosis
cell cycle arrest
wastewater treatment
heavy metals
microbial community
signaling pathway
ionic liquid
oxidative stress
risk assessment
cell death