Disposable aptamer-sensor aided by magnetic nanoparticle enrichment for detection of salivary cortisol variations in obstructive sleep apnea patients.
Renny Edwin FernandezYogeswaran UmasankarPandiaraj ManickamJeffrey C NickelLaura R IwasakiBurt K KawamotoKristen C TodokiJoAnna M ScottShekhar BhansaliPublished in: Scientific reports (2017)
We report a disposable point-of-care sensing platform specific to salivary cortisol detection. The sensor is inkjet printed on a paper substrate with a metalloporphyrin based macrocyclic catalyst ink that can electrochemically reduce cortisol, captured by aptamer functionalized magnetic nanoparticles. The sensor consists of a thin magnet disc, aligned at the back of the electrode, in order to populate the magnetic nanoparticle bound cortisol at the sensing electrode area. Proof of concept studies were performed to detect salivary cortisol levels in human subjects with high and low risks for obstructive sleep apnea (OSA). High selectivity was observed to salivary cortisol against a background of closely related steroids.
Keyphrases
- obstructive sleep apnea
- magnetic nanoparticles
- positive airway pressure
- label free
- end stage renal disease
- gold nanoparticles
- molecularly imprinted
- endothelial cells
- ejection fraction
- chronic kidney disease
- ionic liquid
- real time pcr
- highly efficient
- risk assessment
- room temperature
- high resolution
- amino acid
- carbon dioxide