Selection of Aptamers Against Vimentin for Isolation and Release of Circulating Tumor Cells Undergoing Epithelial Mesenchymal Transition.
Yuan ZhengJialu ZhangMengjiao HuangTeng WangXin QuLingling WuJia SongWei WangYanLing SongChaoyong James YangPublished in: Analytical chemistry (2020)
Circulating tumor cells (CTCs) undergoing epithelial mesenchymal transition (EMT) play an essential role in metastasis and have a better correlation with poor disease outcomes, but the most current affinity-based enrichment methods rely on targeting epithelial markers, which are less effective in capturing CTCs undergoing EMT. Herein, we identified and optimized an aptamer (ZY5C) sequence with high binding affinity and specificity against cell surface vimentin (CSV), which is overexpressed on the post-EMT CTCs. Not only can the hairpin-structured ZY5C aptamer specifically recognize a number of cancer cells with native CSV expression, but it can also bind to CSV expressed on EMT-cells. The Kd value of the ZY5C aptamer against CSV-positive T24 cells was found to be 38 ± 4 nM. Using the evolved ZY5C aptamer and multivalent ZY5C aptamer-functionalized chip, we were able to isolate CTCs from the blood of adenocarcinoma, sarcoma, and carcinosarcoma patients. Overall, this ZY5C aptamer and isolation method bring a fresh approach to CTCs analysis, which not only detects CTCs from nonepithelial origin, but also provides an efficient way to in-depth study the role of post-EMT CTCs in clinical application and metastasis mechanisms.
Keyphrases
- circulating tumor cells
- epithelial mesenchymal transition
- gold nanoparticles
- sensitive detection
- transforming growth factor
- magnetic nanoparticles
- signaling pathway
- circulating tumor
- label free
- end stage renal disease
- cell surface
- quantum dots
- chronic kidney disease
- ejection fraction
- squamous cell carcinoma
- newly diagnosed
- type diabetes
- insulin resistance
- prognostic factors
- peritoneal dialysis
- cell proliferation
- photodynamic therapy
- optical coherence tomography
- metabolic syndrome
- patient reported outcomes
- high throughput
- single cell
- pi k akt
- transcription factor
- mass spectrometry
- patient reported
- cell cycle arrest