Fluorescence Biosensor for Real-Time Interaction Dynamics of Host Proteins with HIV-1 Capsid Tubes.
Derrick LauJames C WalshWang PengVaibhav B ShahStuart TurvilleDavid A JacquesTill BoeckingPublished in: ACS applied materials & interfaces (2019)
The human immunodeficiency virus 1 (HIV-1) capsid serves as a binding platform for proteins and small molecules from the host cell that regulate various steps in the virus life cycle. However, there are currently no quantitative methods that use assembled capsid lattices to measure host-pathogen interaction dynamics. Here we developed a single-molecule fluorescence biosensor using self-assembled capsid tubes as biorecognition elements and imaged capsid binders using total internal reflection fluorescence microscopy in a microfluidic setup. The method is highly sensitive in its ability to observe and quantify binding, to obtain dissociation constants, and to extract kinetics with an extended application of using more complex analytes that can accelerate characterization of novel capsid binders.
Keyphrases
- single molecule
- human immunodeficiency virus
- antiretroviral therapy
- hepatitis c virus
- hiv infected
- living cells
- hiv positive
- atomic force microscopy
- hiv aids
- label free
- high throughput
- life cycle
- high resolution
- single cell
- hiv testing
- quantum dots
- men who have sex with men
- energy transfer
- circulating tumor cells
- cell therapy
- south africa
- bone marrow
- dna binding
- anti inflammatory
- molecularly imprinted
- candida albicans
- high speed