Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector.

Takashi OkumuraYumi HorieChen-Yi LaiHuan-Ting LinHirofumi ShodaBunki NatsumotoKeishi FujioEri KumakiTsubasa OkanoShintaro OnoKay TanitaTomohiro MorioHirokazu KaneganeHisanori HasegawaFumitaka MizoguchiKimito KawahataHitoshi KohsakaHiroshi MoritakeHiroyuki NunoiHironori WakiShin-Ichi TamaruTakayoshi SasakoToshimasa YamauchiTakashi KadowakiHiroyuki TanakaSachiko KitanakaKen NishimuraManami OhtakaMahito NakanishiMakoto Otsu

Published in: Stem cell research & therapy (2019)

This robust and highly efficient method enables the rapid and cost-effective establishment of transgene-free iPSC lines from a small volume of PB, thus facilitating the biobanking of patient-derived iPSCs and their use for the modeling of various diseases.

Keyphrases

highly efficient
peripheral blood
induced apoptosis
cell cycle arrest
induced pluripotent stem cells
case report
heavy metals
endoplasmic reticulum stress
signaling pathway
oxidative stress
pi k akt
nk cells