Silent Death by Sound: C 60 Fullerene Sonodynamic Treatment of Cancer Cells.
Aleksandar RadivoievychBenjamin KolpSergii GrebinykSvitlana PrylutskaUwe RitterOliver ZolkJörn GlöklerMarcus FrohmeAnna GrebinykPublished in: International journal of molecular sciences (2023)
The acoustic pressure waves of ultrasound (US) not only penetrate biological tissues deeper than light, but they also generate light emission, termed sonoluminescence. This promoted the idea of its use as an alternative energy source for photosensitizer excitation. Pristine C 60 fullerene (C 60 ), an excellent photosensitizer, was explored in the frame of cancer sonodynamic therapy (SDT). For that purpose, we analyzed C 60 effects on human cervix carcinoma HeLa cells in combination with a low-intensity US treatment. The time-dependent accumulation of C 60 in HeLa cells reached its maximum at 24 h (800 ± 66 ng/10 6 cells). Half of extranuclear C 60 is localized within mitochondria. The efficiency of the C 60 nanostructure's sonoexcitation with 1 MHz US was tested with cell-based assays. A significant proapoptotic sonotoxic effect of C 60 was found for HeLa cells. C 60 's ability to induce apoptosis of carcinoma cells after sonoexcitation with US provides a promising novel approach for cancer treatment.
Keyphrases
- cell cycle arrest
- induced apoptosis
- cell death
- pi k akt
- endoplasmic reticulum stress
- photodynamic therapy
- oxidative stress
- signaling pathway
- gene expression
- squamous cell carcinoma
- computed tomography
- endothelial cells
- cell proliferation
- stem cells
- bone marrow
- high throughput
- young adults
- replacement therapy
- combination therapy
- papillary thyroid