Real-Time Single-Walled Carbon Nanotube-Based Fluorescence Imaging Improves Survival after Debulking Surgery in an Ovarian Cancer Model.
Lorenzo CeppiNeelkanth M BardhanYoungJeong NaAndrew SiegelNandini RajanRobert FruscioMarcela G Del CarmenAngela M BelcherMichael J BirrerPublished in: ACS nano (2019)
Improved cytoreductive surgery for advanced stage ovarian cancer (OC) represents a critical challenge in the treatment of the disease. Optimal debulking reaching no evidence of macroscopic disease is the primary surgical end point with a demonstrated survival advantage. Targeted molecule-based fluorescence imaging offers complete tumor resection down to the microscopic scale. We used a custom-built reflectance/fluorescence imaging system with an orthotopic OC mouse model to both quantify tumor detectability and evaluate the effect of fluorescence image-guided surgery on post-operative survival. The contrast agent is an intraperitoneal injectable nanomolecular probe, composed of single-walled carbon nanotubes, coupled to an M13 bacteriophage carrying a modified peptide binding to the SPARC protein, an extracellular protein overexpressed in OC. The imaging system is capable of detecting a second near-infrared window fluorescence (1000-1700 nm) and can display real-time video imagery to guide intraoperative tumor debulking. We observed high microscopic tumor detection with a pixel-limited resolution of 200 μm. Moreover, in a survival-surgery orthotopic OC mouse model, we demonstrated an increased survival benefit for animals treated with fluorescence image-guided surgical resection compared to standard surgery.
Keyphrases
- fluorescence imaging
- minimally invasive
- coronary artery bypass
- photodynamic therapy
- mouse model
- surgical site infection
- neoadjuvant chemotherapy
- single molecule
- squamous cell carcinoma
- walled carbon nanotubes
- percutaneous coronary intervention
- magnetic resonance imaging
- computed tomography
- small molecule
- coronary artery disease
- drug delivery
- radiation therapy
- free survival
- atrial fibrillation
- binding protein
- quantum dots
- combination therapy
- replacement therapy