Urinary metabolomic profiling of asthmatics can be related to clinical characteristics.
Claudia Chaves LoureiroA S OliveiraM SantosA RudnitskayaA Todo-BomJ BousquetS M RochaPublished in: Allergy (2016)
Metabolomics has been increasingly explored to achieve an improved understanding of asthma. In the current observational and exploratory study, the first to have examined the relationship between oxidative stress extension, eosinophilic inflammation, and disease severity in asthmatic patients, metabolomics (using target aliphatic aldehydes and alkanes) was carried out using solid-phase microextraction (SPME) followed by a comprehensive two-dimensional gas chromatography coupled to mass spectrometry with a high-resolution time-of-flight analyzer (GC×GC-ToFMS). We were able to demonstrate that metabolomics can give valuable insights into asthma mechanisms once lipidic peroxidation assessed by urinary metabolomics is related to the clinical characteristics of nonobese asthmatics, such as disease severity, lung function, and eosinophilic inflammation. Nevertheless, considering our sample size, the obtained results require further validation using a much larger sample cohort.
Keyphrases
- mass spectrometry
- gas chromatography
- lung function
- oxidative stress
- high resolution
- liquid chromatography
- chronic obstructive pulmonary disease
- cystic fibrosis
- air pollution
- high resolution mass spectrometry
- tandem mass spectrometry
- capillary electrophoresis
- gas chromatography mass spectrometry
- high performance liquid chromatography
- ejection fraction
- end stage renal disease
- newly diagnosed
- peritoneal dialysis
- diabetic rats
- simultaneous determination
- ischemia reperfusion injury
- chronic rhinosinusitis
- single cell
- dna damage
- induced apoptosis
- ms ms
- signaling pathway