Quantitative determination of human IgA subclasses and their Fc-glycosylation patterns in plasma by using a peptide analogue internal standard and ultra-high-performance liquid chromatography/triple quadrupole mass spectrometry.
Hsiao-Fan ChenChing-Ya ShiaoMei-Yi WuYen-Chung LinHsi-Hsien ChenWei-Chiao ChangMai-Szu WuChih-Chin KaoI-Lin TsaiPublished in: Rapid communications in mass spectrometry : RCM (2020)
The developed method showed good validation results, and the absolute quantification results of IgA correlated with those from ELISA. The pilot application study showed that IgA and IgA-glycopeptides can be potential biomarker candidates for kidney diseases, and more clinical sample applications are worth investigating.