Alliacane-Type Secondary Metabolites from Submerged Cultures of the Basidiomycete Clitocybe nebularis .

Seven sesquiterpenoids, named nebucanes A-G ( 1 - 7 ), featuring a rare alliacane scaffold with unprecedented furan or pyrrole functions, were isolated from the fermentation broth of Clitocybe nebularis . Their structures were established on the basis of 1D/2D NMR spectroscopic analyses, HR-(+)-ESIMS spectra, and comparison of measured and calculated CD spectra for determination of the absolute configuration. Assessing the biological activities, nebucane D ( 4 ) exhibited antifungal effects against Rhodotorula glutinis , while nebucane G ( 7 ) displayed significant cytotoxicity against MCF-7 and A431 cell lines.