Overview of the Maintenance of Authentic Cancer Cell Lines.

The proper maintenance of cancer cell lines is critical to maintain the integrity of any experiment. Proper maintenance includes handling of cell lines and maintaining records on usage and confirmation of identity. Cross-contamination of cancer cell lines has been well documented by the ATCC and must be avoided at all costs. In addition, contamination by Mycoplasma sp. is a well-known issue, which, if left unchecked, can affect all data collected as this contamination can affect cell line metabolism and has uncharacterized effects. Considerations and specific recommendations for the proper maintenance of cancer cell lines are presented here.