A straightforward gel-free proteomics pipeline assisted by liquid isoelectric focusing (OFFGEL) and mass spectrometry analysis to study bovine meat proteome.
Enrique SentandreuClaudia Fuente-GarcíaJosé L NavarroMiguel A SentandreuPublished in: Food science and technology international = Ciencia y tecnologia de los alimentos internacional (2020)
Bovine sarcoplasmic sub-proteome was studied through a straightforward gel-free pipeline supported by liquid isoelectric focusing (OFFGEL) protein fractionation coupled to liquid chromatography-mass spectrometry (LC-MS) analysis. Full-MS and data-dependent MS/MS analyses were simultaneously performed by a conventional three-dimensional ion-trap addressing targeted quantitative and untargeted qualitative research, respectively. There were unambiguously identified 47 proteins distributed along 12 OFFGEL fractions assayed. Regarding intermediate- and high-abundant peptides, bulky quantitative data processing performed by MZmine 2 freeware yielded a satisfactory linearity and coefficient of variation with r2 in the 0.95-0.99 range and about 25%, respectively. Up to 41 peptides from 20 identified proteins were relatively quantified throughout OFFGEL fractions. This reliable, flexible and affordable gel-free proteomic approach could be readily implemented by industry to improve quality assessment of protein-based food products.
Keyphrases
- mass spectrometry
- liquid chromatography
- high resolution
- high resolution mass spectrometry
- gas chromatography
- high performance liquid chromatography
- ms ms
- tandem mass spectrometry
- capillary electrophoresis
- electronic health record
- ultra high performance liquid chromatography
- systematic review
- computed tomography
- hyaluronic acid
- ionic liquid
- binding protein
- magnetic resonance imaging
- magnetic resonance
- wound healing
- protein protein
- small molecule