Near-Infrared Light-Activated DNA-Agonist Nanodevice for Nongenetically and Remotely Controlled Cellular Signaling and Behaviors in Live Animals.
Miao WangFang HeHao LiSihui YangJinghui ZhangPradipta GhoshHong-Hui WangZhou NiePublished in: Nano letters (2019)
Optogenetics provides promising tools for the precise control of receptor-mediated cell behaviors in a spatiotemporal manner. Yet, most photoreceptors require extensive genetic manipulation and respond only to ultraviolet or visible light, which are suboptimal for in vivo applications because they do not penetrate thick tissues. Here we report a novel near-infrared light-activated DNA agonist (NIR-DA) nanodevice for nongenetic manipulation of cell signaling and phenotype in deep tissues. This nanodevice is prepared by conjugating a preinactivated DNA agonist onto the gold nanorods (AuNRs). Upon NIR light treatment, the DNA agonist is released through the localized surface plasmon resonance (LSPR)-based photothermal effect of AuNRs and becomes active. The active DNA agonist dimerizes the DNA-modified chimeric or native receptor tyrosine kinase (RTK) on cell surfaces and activates downstream signal transduction in live cells. Such NIR-DA activation of RTK signaling enables the control of cytoskeletal remodeling, cell polarization, and directional migration. Furthermore, we demonstrate that the NIR-DA system can be used in vivo to mediate RTK signaling and skeletal muscle satellite cell migration and myogenesis, which are critical cellular behaviors in the process of skeletal muscle regeneration. Thus, the NIR-DA system offers a powerful and versatile platform for exogenous modulation of deep tissues for purposes such as regenerative medicine.
Keyphrases
- circulating tumor
- skeletal muscle
- photodynamic therapy
- cell free
- cell therapy
- single molecule
- tyrosine kinase
- single cell
- drug release
- gene expression
- cell migration
- fluorescent probe
- insulin resistance
- epidermal growth factor receptor
- bone marrow
- induced apoptosis
- type diabetes
- high throughput
- staphylococcus aureus
- signaling pathway
- cystic fibrosis
- pi k akt
- endoplasmic reticulum stress
- biofilm formation
- candida albicans
- reduced graphene oxide