A novel label-free, pre-equilibrium assay to determine the association and dissociation rate constants of therapeutic antibodies on living cells.
Eric M JanezicAlexander DoanElaine MaiDaniel D BravoJianyong WangHok Seon KimChristoph SpiessKathryn BewleyAdel ElSohlyWei-Ching LiangJames T KoerberPascale RichaletMarc VanhoveLaetitia Comps-AgrarPublished in: British journal of pharmacology (2023)
Our novel assay has the potential to systematically probe binding kinetics of monoclonal Abs to cells and can be incorporated in a screening cascade to identify new therapeutic candidates. Wide-spread adoption of pre-equilibrium assays using physiologically relevant systems will lead to a more holistic understanding of how Ab binding kinetics influence their potency.
Keyphrases
- living cells
- label free
- fluorescent probe
- high throughput
- aqueous solution
- induced apoptosis
- single molecule
- molecular dynamics
- molecular dynamics simulations
- cell cycle arrest
- dna binding
- binding protein
- cell death
- endoplasmic reticulum stress
- oxidative stress
- single cell
- multiple myeloma
- human health
- signaling pathway
- quantum dots
- transcription factor