Combination of ultrashort PCR and Pyrococcus furiosus Argonaute for DNA detection.

Lixin MaLongyu WangFei WangJun YangXiao YuYuan WangZhiguo LiuChunhua LiLixin Ma

Published in: The Analyst (2021)

A simple and user-friendly nucleic acid sensing platform with 10 aM sensitivity, named USPCRP (combines ultrashort PCR with Pyrococcus furiosus Argonaute cleavage for nuleic acids detection) is reported. The product of this ultrashort PCR could be directly used as a DNA guide to mediate PfAgo cleavage of molecular beacons.

Keyphrases

real time pcr
nucleic acid
loop mediated isothermal amplification
single molecule
dna binding
label free
high throughput
cell free
sensitive detection