A perylenemonoimide-based fluorescent probe: ultrasensitive and selective tracing of endogenous peroxynitrite in living cells.

Aasif KhanVinod Kumar MeenaAkshay Silswal Apurba Lal Koner

Published in: The Analyst (2023)

Peroxynitrite (ONOO - ), a highly reactive species, plays a key role in various physiological and pathological processes. Herein, a red-emitting fluorescent reporter perylenemonoimide-boronate ester (PMI-BE) was synthesized and utilized for ultrasensitive detection of ONOO - . The unique feature of PMI-BE is its nanomolar sensitivity with high selectivity towards ONOO - . Moreover, PMI-BE also detects endogenously generated ONOO - in live cells.

Keyphrases

fluorescent probe
living cells
label free
quantum dots
gold nanoparticles
induced apoptosis
molecularly imprinted
machine learning
cell cycle arrest
crispr cas
endoplasmic reticulum stress
deep learning
single molecule
loop mediated isothermal amplification
signaling pathway
cell proliferation
sensitive detection