Acupotomy Alleviates Energy Crisis at Rat Myofascial Trigger Points.
Yi ZhangNing-Yu DuChen ChenTong WangLi-Juan WangXiao-Lu ShiShu-Ming LiChang-Qing GuoPublished in: Evidence-based complementary and alternative medicine : eCAM (2020)
The aim of this study was to determine the effects of acupotomy on energy crises in rat trigger points (TrPs) by measuring mechanical pain thresholds (MPTs) and levels of acetylcholinesterase (AChE), free sarcoplasmic calcium (Ca2+), adenosine 5'-triphosphate (ATP), adenosine 5'-monophosphate (AMP), substance P (SP), and calcitonin gene-related peptide (CGRP) in rat muscle TrP tissue. Male Sprague Dawley rats (n = 32) were randomly divided into four groups: control, TrP, acupotomy, and lidocaine injection. Enzyme-linked immunosorbent assays were used to measure AChE, and free sarcoplasmic Ca2+ concentrations were determined by fluorescent staining with Fura-2 AM; high-performance liquid chromatography was used to measure ATP and AMP, and SP and CGRP were evaluated by immunohistochemistry. Compared with the control group, free sarcoplasmic Ca2+, AMP, SP, and CGRP were higher in the model group, while MPT, AChE, and ATP were lower. Treatment with acupotomy or lidocaine injection reduced free sarcoplasmic Ca2+, SP, and CGRP and increased MPTs and AChE levels compared with the model group. However, only acupotomy also led to decreased AMP and increased ATP levels relative to the model group. We conclude that acupotomy can alleviate energy crises at TrPs.
Keyphrases
- protein kinase
- high performance liquid chromatography
- oxidative stress
- chronic pain
- public health
- skeletal muscle
- quantum dots
- tandem mass spectrometry
- ultrasound guided
- neuropathic pain
- high throughput
- gene expression
- simultaneous determination
- copy number
- ms ms
- mouse model
- high resolution
- smoking cessation
- label free
- drug induced