Real-Time Intracellular Measurements of ROS and RNS in Living Cells with Single Core-Shell Nanowire Electrodes.
Xin-Wei ZhangQuan-Fa QiuHong JiangFu-Li ZhangYan-Lin LiuChristian AmatoreWei-Hua HuangPublished in: Angewandte Chemie (International ed. in English) (2017)
Nanoelectrodes allow precise and quantitative measurements of important biological processes at the single living-cell level in real time. Cylindrical nanowire electrodes (NWEs) required for intracellular measurements create a great challenge for achieving excellent electrochemical and mechanical performances. Herein, we present a facile and robust solution to this problem based on a unique SiC-core-shell design to produce cylindrical NWEs with superior mechanical toughness provided by the SiC nano-core and an excellent electrochemical performance provided by the ultrathin carbon shell that can be used as such or platinized. The use of such NWEs for biological applications is illustrated by the first quantitative measurements of ROS/RNS in individual phagolysosomes of living macrophages. As the shell material can be varied to meet any specific detection purpose, this work opens up new opportunities to monitor quantitatively biological functions occurring inside cells and their organelles.
Keyphrases
- living cells
- reactive oxygen species
- gold nanoparticles
- label free
- reduced graphene oxide
- fluorescent probe
- dna damage
- cell death
- induced apoptosis
- high resolution
- room temperature
- single cell
- stem cells
- molecularly imprinted
- signaling pathway
- solid state
- metal organic framework
- mass spectrometry
- highly efficient
- oxidative stress
- carbon nanotubes
- loop mediated isothermal amplification
- high efficiency