Evaluation of the BD Phoenix Carbapenemase-Producing Organism Panels for the Detection of Carbapenemase Producers in Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa .

The classification of carbapenemases can help guide therapy. The present study evaluated the performance of the CPO detection test, included in the BD Phoenix ™ NMIC-501 panel for the detection and classification of carbapenemases on the representative molecularly characterized strains collection from Mexico. Carbapenem non-susceptible isolates collected in Mexico were included. The clinical isolates ( n = 484) comprised Klebsiella pneumoniae ( n = 154), Escherichia coli ( n = 150), and P. aeruginosa ( n = 180). BD Phoenix CPO NMIC-504 and NMIC-501 panels were used for the identification of species, antimicrobial susceptibility tests, and detection of CPOs. For the detection of carbapenemase-encoding genes, E. coli and K. pneumoniae were evaluated using PCR assays for bla NDM-1 , bla KPC , bla VIM , bla IMP , and bla OXA-48-like . For P. aeruginosa , bla VIM, bla IMP, and bla GES were detected using PCR. Regarding E. coli , the CPO panels had a sensitivity of 70% and specificity of 83.33% for the detection of a class B carbapenemase ( bla NDM in the molecular test). Regarding K. pneumoniae , the panels had a sensitivity of 75% and specificity of 100% for the detection of a class A carbapenemase ( bla KPC in the molecular test). The Phoenix NMIC-501 panels are reliable for detecting class B carbapenemases in E. coli . The carbapenemase classification in K. pneumoniae for class A carbapenemases has a high specificity and PPV; thus, a positive result is of high value.