MDM2-Associated Clusterization-Triggered Emission and Apoptosis Induction Effectuated by a Theranostic Spiropolymer.
Pai LiuWeiqiang FuPeter VerwilstMiae WonJinwoo ShinZhengxu CaiBin TongJianbing ShiYuping DongJong Seung KimPublished in: Angewandte Chemie (International ed. in English) (2020)
Heteroatom-containing spiropolymers were constructed in a facile manner by a catalyst-free multicomponent spiropolymerization route. P1a2b as the most potent of these spiropolymers, demonstrates cluster-triggered emission resulting from strong interactions with the MDM2 protein. By preventing the anti-apoptotic p53/MDM2 interaction, P1a2b triggers apoptosis in cancerous cells, while demonstrating a good biocompatibility and non-toxicity in non-cancerous cells. The combined results from solution and cell-based cluster-triggered emission studies, docking, protein expression experiments and cytotoxicity data strongly support the MDM2-binding hypothesis and indicate a potential application as a fluorescent cancer marker as well as therapeutic for this spiropolymer.
Keyphrases
- cell cycle arrest
- cell death
- induced apoptosis
- endoplasmic reticulum stress
- oxidative stress
- pi k akt
- quantum dots
- reduced graphene oxide
- highly efficient
- papillary thyroid
- squamous cell carcinoma
- room temperature
- big data
- cell therapy
- binding protein
- wastewater treatment
- stem cells
- machine learning
- bone marrow
- fluorescence imaging
- anti inflammatory
- dna binding
- artificial intelligence
- metal organic framework
- deep learning
- living cells
- small molecule
- visible light