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Towards a unified protocol for handling of CSF before β-amyloid measurements.

Shorena JanelidzeErik StomrudBritta BrixOskar Hansson
Published in: Alzheimer's research & therapy (2019)
CSF can be stored for up to 72 h at RT, 1 week at 4 °C, or at least 2 weeks at either - 20 °C or - 80 °C before Aβ measurements. Centrifugation of fresh non-blood-contaminated CSF after LP, or mixing before analysis, is not required. In case of visible blood contamination, centrifugation and storage at 4 °C or - 20 °C is recommended. After discarding the first 2 ml, any portion of up to 20 ml of CSF is suitable for Aβ analysis. These findings will be important for the development of a clinical routine protocol for pre-analytical handling of CSF.
Keyphrases
  • cerebrospinal fluid
  • drinking water
  • risk assessment
  • clinical trial
  • mass spectrometry
  • clinical practice
  • climate change
  • human health
  • placebo controlled
  • data analysis