Precision in cancer diagnostics: ultra-sensitive detection of MCF-7 breast cancer cells by gold nanostructure-enhanced electrochemical biosensing.
Mahsa RahmanipourHossein SiampourAhmad MoshaiiMasoud AmirabadizadehMohamad Hassan FouaniLaleh ShariatiMohammad RafieniaPublished in: Journal of materials chemistry. B (2024)
Timely identification of cancers is pivotal in optimizing treatment efficacy and reducing their widespread impact. This study introduces a novel biosensor for the sensitive electrochemical detection of cancer cells overexpressing mucin 1 (MUC1), a well-established model for breast cancer. The sensor substrate comprises gold columnar nanostructures obtained through glancing angle deposition (GLAD) of copper nanostructures, subsequently replaced by gold via a facile galvanic replacement process. Functionalizing these gold nanostructures with aptamers targeting the MUC1 glycoproteins, a prominent cancer biomarker, enables specific recognition of MCF-7 breast cancer cells. The proposed electrochemical sensing platform offers several advantages, including high selectivity, a wide linear range of detection, a low detection limit of 30 cells per mL, and long-term stability, rendering this sensor highly desirable for definitive breast cancer diagnosis.
Keyphrases
- label free
- breast cancer cells
- sensitive detection
- loop mediated isothermal amplification
- gold nanoparticles
- papillary thyroid
- quantum dots
- silver nanoparticles
- induced apoptosis
- high resolution
- squamous cell
- real time pcr
- molecularly imprinted
- signaling pathway
- cancer therapy
- oxidative stress
- cell cycle arrest
- rectal cancer
- reduced graphene oxide
- cell proliferation