Three-dimensional super-resolution fluorescence imaging of DNA.
Sevim YardimciDaniel R BurnhamSamantha Y A TerryHasan YardimciPublished in: Scientific reports (2020)
Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage of bis- over mono-intercalators before demonstrating the approach by visualizing single DNA molecules stretched between microspheres at various heights. Finally, the approach is applied to the more complex environment of intact and damaged metaphase chromosomes, unravelling their structural features.
Keyphrases
- single molecule
- circulating tumor
- living cells
- cell free
- high resolution
- fluorescence imaging
- high speed
- nucleic acid
- optical coherence tomography
- high throughput
- photodynamic therapy
- binding protein
- deep learning
- quantum dots
- ionic liquid
- machine learning
- circulating tumor cells
- transcription factor
- mass spectrometry
- convolutional neural network
- crystal structure