Login / Signup

Genome-scale chromatin binding dynamics of RNA Polymerase II general transcription machinery components.

Kristyna KupkovaSavera J ShettyElizabeth A HoffmanStefan BekiranovDavid T Auble
Published in: The EMBO journal (2024)
A great deal of work has revealed, in structural detail, the components of the preinitiation complex (PIC) machinery required for initiation of mRNA gene transcription by RNA polymerase II (Pol II). However, less-well understood are the in vivo PIC assembly pathways and their kinetics, an understanding of which is vital for determining how rates of in vivo RNA synthesis are established. We used competition ChIP in budding yeast to obtain genome-scale estimates of the residence times for five general transcription factors (GTFs): TBP, TFIIA, TFIIB, TFIIE and TFIIF. While many GTF-chromatin interactions were short-lived ( < 1 min), there were numerous interactions with residence times in the range of several minutes. Sets of genes with a shared function also shared similar patterns of GTF kinetic behavior. TFIIE, a GTF that enters the PIC late in the assembly process, had residence times correlated with RNA synthesis rates. The datasets and results reported here provide kinetic information for most of the Pol II-driven genes in this organism, offering a rich resource for exploring the mechanistic relationships between PIC assembly, gene regulation, and transcription.
Keyphrases
  • transcription factor
  • genome wide
  • genome wide identification
  • dna binding
  • dna methylation
  • gene expression
  • dna damage
  • healthcare
  • rna seq
  • circulating tumor cells
  • genome wide analysis
  • oxidative stress
  • nucleic acid